SOX10 anticorps (AA 115-269)

N° du produit ABIN5707896

L’anticorps Lapin Monoclonal anti-SOX10 a été validé pour IHC (p). Il convient pour détecter SOX10 dans des échantillons de Humain.

Aperçu rapide pour SOX10 anticorps (AA 115-269) (ABIN5707896)

Antigène: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX10 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SOX10-2311R

Détail du produit anti-SOX10 anticorps

Épitope: AA 115-269

Purification: Purified

Pureté: Protein A affinity chromatography

Immunogène: Amino acids 115-269 of the human protein were used as the immunogen for the recombinant SOX10 antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the recombinant SOX10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant SOX10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur SOX10

Antigène: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Autre désignation: SOX10

Sujet: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Pathways: Chromatin Binding